New Step by Step Map For high performance liquid chromatography
New Step by Step Map For high performance liquid chromatography
Blog Article
HPLC works following The fundamental theory of thin layer chromatography or column chromatography, where by it's got a stationary period along with a cell period. The cellular stage flows through the stationary period and carries the elements on the mixture with it.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
Just before using a mobile section solvent we must get rid of dissolved gases, which include N2 and O2, and smaller particulate make a difference, such as dust. Mainly because You will find a substantial drop in force through the column—the force in the column’s entrance is as much as several hundred atmospheres, however it is atmospheric strain on the column’s exit—gases dissolved while in the mobile period are introduced as gas bubbles which could interfere Along with the detector’s reaction.
. When we take a look at the chromatograms from these seven cell phases we might notice that one or more offers an suitable separation, or we might discover a location throughout the solvent triangle in which a separation is feasible.
The data acquisition system records and analyses the detector indicators, permitting substances to become quantified based mostly on their peak regions in the chromatogram.
Fluoxetine is another identify for your antidepressant drug Prozac. The determination of fluoxetine in serum is a crucial Element of monitoring its therapeutic use.
各種の高速液体クロマトグラフィーの項目にある違いは、カラムの違いである事が多いため、装置はそのままでカラムの変更で行える場合が有る。ただし、誤って不適当な溶媒を通すとカラムを破損することがあるため、切り替えを行う際には注意が必要である。
Acid–base chemistry isn't the only illustration of a secondary equilibrium response. Other examples include things like ion-pairing, complexation, plus the interaction of solutes with micelles. We'll take into account the last of these in Chapter 12.7 whenever we examine micellar electrokinetic capillary chromatography.
-hydroxybenzoic acid—on a nonpolar C18 column employing an aqueous buffer of acetic acid and sodium acetate because the cell phase. The retention periods for these weak acids are shorter when utilizing a considerably less acidic mobile period for the reason that Just about every solute is existing in an anionic, weak base sort which is a lot less soluble during the nonpolar stationary phase.
High-performance liquid chromatography (HPLC) is a strong analytical technique for separating high performance liquid chromatography and identifying elements in a mixture. Acquiring precise and trusted outcomes calls for thorough attention to each stage in the Investigation, from sample preparing to details interpretation.
Fluoxetine is an additional name for your antidepressant drug Prozac. The determination of fluoxetine in serum is an important part of checking its therapeutic use.
Solvent composition: The ratio of solvents inside the mobile phase is usually great-tuned to boost peak resolution and separation.
특히 컬럼의 선정은 분석의 결과에 영향을 미치기에 신중하게 선택하여야 합니다.
The injector introduces a precise quantity of your sample Answer into the cell period stream. high performance liquid chromatography Numerous injection techniques exist, with loop injection becoming a standard approach.